survivalsetr.blogg.se - Palindromic sequence recognized by restriction enzymes

Not sure why Larry Parnell was down-voted, he was not technically wrong.Ĭrystal structures of the most popular restriction enzymes are already known and can easily be found the Protein Data Bank or Wikipedia for graphical reference. If you look at the histogram of lengths, there is no bias towards even recognition site lengths except for length 6. Ggplot(df, aes(x=mids, y=counts, fill=even))+geom_bar(stat="identity")

Removed all duplicate recognition sites leaving 465 distinct recognition sequences ( my fault for not removing them in the first instance).

Expanded dataset to include new recognition sites from the resource that 96well linked to.

H0 rejected with 95% confidence (indeed with 99.9%+ confidence)Ĭan anyone explain or suggest why it is more common that restriction enzymes recognition sites have an even number of bases? Updates

P=0.05, 1 Degree of Freedom: Critical Value of 3.841

There is no significant difference between the number of restriction sequences The results are probably best summarised graphically: I wondered if this was just a co-incidence, so I took the data from this site for over a thousand known recognition sites and put it into a spreadsheet ( XLS uploaded here). When reading my textbook I noticed that in all examples but one from eight the recognition site was an even number of bases.